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Immunohistochemistry (IHC) is a useful method to detect and visualize targeted proteins in tissues, with a high degree of specificity and clarity. Through the strategic use of chromogens and counterstains, it enables a contrast-rich and accurate evaluation of specific molecules, such as surface markers. Here, we outline a comprehensive IHC followed with counterstaining protocol for the positive selection of stem-like cells and immune cells in brain tumor tissue.

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Unmasking Stemness and Immune Cell Markers in Brain Tumors with Immunohistochemistry

  • 实验方案

  • 发表日期：

    2025年

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  • Jack Q. Lu,
  • Kui Zhai,
  • Agata M. Kieliszek,
  • Chitra Venugopal,
  • Sheila K. Singh

摘要

Immunohistochemistry (IHC) is a useful method to detect and visualize targeted proteins in tissues, with a high degree of specificity and clarity. Through the strategic use of chromogens and counterstains, it enables a contrast-rich and accurate evaluation of specific molecules, such as surface markers. Here, we outline a comprehensive IHC followed with counterstaining protocol for the positive selection of stem-like cells and immune cells in brain tumor tissue.