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CRISPR-Cas9 technology has revolutionized scientific research and has provided scientists with the ability to change DNA bases specifically and precisely at predetermined sites. The CRISPR-Cas9 knockout (KO) and activation (a) platforms developed by Hart et al. and Sanson et al. allow for RNA-directed genome editing to both decrease or increase gene expression, respectively [1, 2]. In this chapter the two techniques, CRISPR KO and CRISPRa, will be discussed and explained in further detail for optimized application against patient derived brain tumor cells.

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CRISPR-Cas9-Guided Genetic Manipulation of Patient-Derived Brain Tumor Cells

  • 实验方案

  • 发表日期：

    2025年

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  • Yujin Suk,
  • Erika Apel,
  • Stefan Custers,
  • Petar Miletic,
  • Kui Zhai,
  • Chirayu Chokshi,
  • Chitra Venugopal,
  • Jason Moffat,
  • Sheila K. Singh

摘要

CRISPR-Cas9 technology has revolutionized scientific research and has provided scientists with the ability to change DNA bases specifically and precisely at predetermined sites. The CRISPR-Cas9 knockout (KO) and activation (a) platforms developed by Hart et al. and Sanson et al. allow for RNA-directed genome editing to both decrease or increase gene expression, respectively [1, 2]. In this chapter the two techniques, CRISPR KO and CRISPRa, will be discussed and explained in further detail for optimized application against patient derived brain tumor cells.