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The process of extracting genomic DNA from biological samples is a bottleneck in PCR genotyping. Here, we describe a speedy genotyping method that combines simple genomic DNA extraction using hot alkaline/SDS and direct use of the extract in an SDS-tolerant cyclodextrin-PCR (CD-PCR).

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High-Throughput Genotyping by CD-PCR

  • 实验方案

  • 发表日期：

    2025年

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  • Yoichi Nakanishi,
  • Shunta Taga

摘要

The process of extracting genomic DNA from biological samples is a bottleneck in PCR genotyping. Here, we describe a speedy genotyping method that combines simple genomic DNA extraction using hot alkaline/SDS and direct use of the extract in an SDS-tolerant cyclodextrin-PCR (CD-PCR).