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The interplay between viruses and host innate immunity is very intricate, with viral proteins having evolved various strategies to target multiple steps of antiviral innate immune signaling pathways. The dual-luciferase reporter assay is a high-flux and efficient method that can rapidly screen potential viral immune evasion components. In this context, a dual-luciferase reporter assay is applied to screen and identify viral proteins that target the host signaling pathway for immune evasion, which provides valuable insights into the function of viral proteins during infections.

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Dual-Luciferase Reporter Assay to Investigate Virus–Host Interactions

  • 实验方案

  • 发表日期：

    2025年

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  • Li Yi,
  • Chunfu Zheng

摘要

The interplay between viruses and host innate immunity is very intricate, with viral proteins having evolved various strategies to target multiple steps of antiviral innate immune signaling pathways. The dual-luciferase reporter assay is a high-flux and efficient method that can rapidly screen potential viral immune evasion components. In this context, a dual-luciferase reporter assay is applied to screen and identify viral proteins that target the host signaling pathway for immune evasion, which provides valuable insights into the function of viral proteins during infections.