Western blotting (WB) is an end-point technique that is used to analyze the expression of target proteins in cells and tissues. It involves the separation of proteins using SDS-PAGE followed by an electrophoretic transfer of the proteins from the gel to a hydrophobic membrane where they will be immunodetected and captured on a film or by an imager. The peroxisome proliferator-activated receptor gamma (PPARγ) is a type of nuclear receptor protein that plays a crucial role in regulating gene expression and various cellular functions. In this chapter, the western blot procedure is demonstrated for the detection and quantification of the PPARγ nuclear protein from either adipocyte cell lines or adipose tissue.