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Wharton’s jelly-derived mesenchymal stromal/stem cells (WJSCs) are growing in popularity as an option for cell-based therapies. These cells are found within the gelatinous matrix of the umbilical cord, referred to as Wharton’s jelly. WJSCs can be isolated by enzymatic tissue digestion or by using the explant method. Here, we discuss the explant method for the isolation of WJSCs.

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Isolation of Wharton’s Jelly-Derived Mesenchymal Stromal/Stem Cells

  • 实验方案

  • 发表日期：

    2025年

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  • Carina C. Babbo,
  • Chrisna Durandt,
  • Melvin A. Ambele,
  • Michael S. Pepper

摘要

Wharton’s jelly-derived mesenchymal stromal/stem cells (WJSCs) are growing in popularity as an option for cell-based therapies. These cells are found within the gelatinous matrix of the umbilical cord, referred to as Wharton’s jelly. WJSCs can be isolated by enzymatic tissue digestion or by using the explant method. Here, we discuss the explant method for the isolation of WJSCs.