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Adipogenesis is a complex, multistep process by which precursor cells such as mesenchymal stromal/stem cells (MSCs) differentiate into mature adipocytes. The ability of MSCs to differentiate into adipocytes serves as a model to study human adipogenesis in vitro. Flow cytometry is a powerful technique that can be used to analyze the properties of individual cells. In this chapter, we describe a flow cytometry-based technique to quantify the proportion of MSCs that have differentiated into adipocytes.

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Quantification of Adipogenesis Using Flow Cytometry

  • 实验方案

  • 发表日期：

    2025年

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  • Carina C. Babbo,
  • Chrisna Durandt,
  • Melvin A. Ambele,
  • Michael S. Pepper

摘要

Adipogenesis is a complex, multistep process by which precursor cells such as mesenchymal stromal/stem cells (MSCs) differentiate into mature adipocytes. The ability of MSCs to differentiate into adipocytes serves as a model to study human adipogenesis in vitro. Flow cytometry is a powerful technique that can be used to analyze the properties of individual cells. In this chapter, we describe a flow cytometry-based technique to quantify the proportion of MSCs that have differentiated into adipocytes.