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Adipose-derived stromal/stem cells (ASCs) have generated great interest both with regard to research and their potential applications in translational clinical sciences. ASCs are often required for use at a later time relative to the time at which they were harvested. This requires appropriate conditions and procedures for storage and retrieval of these cells as needed. This protocol describes cryopreservation and thawing of ASCs without compromising the viability of the stored cells.

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Freezing and Thawing of Adipose-Derived Stromal/Stem Cells (ASCs)

  • 实验方案

  • 发表日期：

    2025年

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  • Ladislaus Tapiwa Adam,
  • Michael S. Pepper,
  • Melvin A. Ambele

摘要

Adipose-derived stromal/stem cells (ASCs) have generated great interest both with regard to research and their potential applications in translational clinical sciences. ASCs are often required for use at a later time relative to the time at which they were harvested. This requires appropriate conditions and procedures for storage and retrieval of these cells as needed. This protocol describes cryopreservation and thawing of ASCs without compromising the viability of the stored cells.