Scholarlink

Human mesenchymal stem/stromal cells (hMSCs) constitute a primary cell source in adipogenesis, playing a pivotal role in the generation of new adipocytes. This process is accompanied by substantial metabolic shifts in hMSCs, notably characterized by a transition from glycolytic to oxidative metabolism. Monitoring metabolic alterations during adipogenic differentiation can be achieved through the assessment of extracellular flux parameters, such as the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). In this study, we provide a comprehensive protocol for differentiating hMSCs into the adipogenic lineage, with an emphasis on tracking differentiation stages through extracellular flux measurements. We determined the cellular metabolic status throughout the adipogenic differentiation by assessing OCR and ECAR to gauge mitochondrial respiration and glycolytic activity, respectively, using a Seahorse XFe96 extracellular flux analyzer.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Oxygen Consumption and Extracellular Acidification Rate in Adipogenic Differentiated Human Mesenchymal Stromal/Stem Cells

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Miguel Angel Olmedo-Suárez,
  • Edgar Sánchez-Ramírez,
  • Ximena del Toro-Rios,
  • Lilia G. Noriega,
  • Lorena Aguilar-Arnal

摘要

Human mesenchymal stem/stromal cells (hMSCs) constitute a primary cell source in adipogenesis, playing a pivotal role in the generation of new adipocytes. This process is accompanied by substantial metabolic shifts in hMSCs, notably characterized by a transition from glycolytic to oxidative metabolism. Monitoring metabolic alterations during adipogenic differentiation can be achieved through the assessment of extracellular flux parameters, such as the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). In this study, we provide a comprehensive protocol for differentiating hMSCs into the adipogenic lineage, with an emphasis on tracking differentiation stages through extracellular flux measurements. We determined the cellular metabolic status throughout the adipogenic differentiation by assessing OCR and ECAR to gauge mitochondrial respiration and glycolytic activity, respectively, using a Seahorse XFe96 extracellular flux analyzer.