Protein microarrays are powerful proteomics tools for high-throughput analysis. They allow to screen simultaneously large numbers of proteins for their binding specificity, enzymatic activity, and abundance in biological samples, among other uses. One widely used platform for protein microarray analysis is GenePix, which offers instrumentation and software solutions for data acquisition and analysis. Here, we provide an overview of the use of GenePix for protein microarray analysis, exploring its applications and advantages.