Attana has developed a high-class quartz crystal microbalance (QCM) technology that allows to measure the kinetics of molecular interactions. The technology is versatile, allowing biochemical, cell-based, and tissue-based assays using any kind of biological fluid and measuring in real time and label-free. Here, we present the methods to study the molecular interactions of analytes in cell-based assays (both adherent and suspension cells), where the objective is to provide more biologically relevant and highly informative data than obtained in biochemical assays using purified molecules.

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Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors

  • Claudia Durall,
  • Ahmed Ibrahim,
  • Teodor Aastrup

摘要

Attana has developed a high-class quartz crystal microbalance (QCM) technology that allows to measure the kinetics of molecular interactions. The technology is versatile, allowing biochemical, cell-based, and tissue-based assays using any kind of biological fluid and measuring in real time and label-free. Here, we present the methods to study the molecular interactions of analytes in cell-based assays (both adherent and suspension cells), where the objective is to provide more biologically relevant and highly informative data than obtained in biochemical assays using purified molecules.