Competitive epitope binning using high-throughput surface plasmon resonance (HT-SPR) is a method for understanding competitive epitope profiles of up to hundreds of monoclonal antibodies. Detailed here is an approach along with considerations for the design and analysis of a classical competitive epitope binning assay using the Carterra LSA. Monoclonal antibodies are arrayed on a biosensor chip and interrogated serially for their ability to form a trimolecular complex with antigen and solution phase monoclonal antibodies. Software tools allow the clustering of monoclonal antibodies sharing common epitopes, and the real-time nature of the data allows for study of binding profiles for each monoclonal antibody.