Scholarlink

Peptide nucleic acids (PNA) [1] are synthetic DNA analogs that are used as DNA/RNA mimics for diagnostic [2, 3] and therapeutic purposes [2–5]. One sensing strategy is the use of fluorogenic probes. One such probe is forced intercalation (FIT) PNA, which has a surrogate base as a cyanine dye (e.g., BisQ, TO) that replaces one of the natural bases in the PNA sequence. FIT-PNAs fluoresce after DNA/RNA hybridization, and display sensitivity towards single base mismatches [6]. These, combined with other desirable features, render them highly attractive as RNA/DNA sensors. Herein, we describe the procedure for FIT-PNA synthesis, both by manual and automated methodologies.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Fmoc Solid-Phase FIT-PNA Synthesis—Manual and Automated Methodologies

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Huda Nazzal,
  • Eylon Yavin

摘要

Peptide nucleic acids (PNA) [1] are synthetic DNA analogs that are used as DNA/RNA mimics for diagnostic [2, 3] and therapeutic purposes [2–5]. One sensing strategy is the use of fluorogenic probes. One such probe is forced intercalation (FIT) PNA, which has a surrogate base as a cyanine dye (e.g., BisQ, TO) that replaces one of the natural bases in the PNA sequence. FIT-PNAs fluoresce after DNA/RNA hybridization, and display sensitivity towards single base mismatches [6]. These, combined with other desirable features, render them highly attractive as RNA/DNA sensors. Herein, we describe the procedure for FIT-PNA synthesis, both by manual and automated methodologies.