Synthesis of glycopeptide libraries is often limited by the cost and availability of the glycoamino acids. To circumvent this, we have devised a method of using SPOT synthesis for production of such libraries using cellulose filter paper as the solid support. We termed this method Glyco-SPOT synthesis, and it can be used to produce libraries of glycopeptides in microgram quantities in a parallel manner using microgram amounts of glycoamino acids. This chapter provides details on the method to perform this synthesis process.