Peptide nanomaterials are a class of materials characterized by high biocompatibility, excellent degradability, and unique biomimetic properties. Moreover, they feature tunable amino acid structures and sequences, allowing flexible design and customization. By appropriately adjusting external factors such as solvents, pH, and temperature, peptide molecules can self-assemble into nanostructures ranging from zero-dimensional to three-dimensional forms driven by non-covalent interactions. Through the modification of functional groups abundant on peptides, these molecules can combine with nanoparticles, drug molecules, 2D materials, and other entities to form hybrid peptide-based nanomaterials with specific functionalities, which hold significant potential for applications in medicine, biotechnology, and environmental science. In this chapter, we provide key information and details for the synthesis of various hybrid peptide-based nanomaterials, which could be useful for readers to understand the design principles and synthesis strategies.