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The bacterial rapid approach to DNA adduct recovery (RADAR) assay allows for the rapid lysis and trapping of DNA-topoisomerase cleavage complexes, as well as other proteins covalently attached to DNA. These covalently attached proteins can be quantified when the isolated DNA is immunoblotted. Significantly, the bacterial RADAR assay described here requires no large, expensive lab equipment, thus making it accessible to most laboratories. Furthermore, it does not require any long centrifugation steps, which allows for a relatively large number of samples to be processed in 1 day.

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Bacterial RADAR Assay: Isolation and Detection of Intracellular Covalent Complexes

  • 实验方案

  • 发表日期：

    2025年

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  • Katie J. Aldred

摘要

The bacterial rapid approach to DNA adduct recovery (RADAR) assay allows for the rapid lysis and trapping of DNA-topoisomerase cleavage complexes, as well as other proteins covalently attached to DNA. These covalently attached proteins can be quantified when the isolated DNA is immunoblotted. Significantly, the bacterial RADAR assay described here requires no large, expensive lab equipment, thus making it accessible to most laboratories. Furthermore, it does not require any long centrifugation steps, which allows for a relatively large number of samples to be processed in 1 day.