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Limited literature on the optimal chromatographic method for cerebrospinal fluid (CSF) extraction and identification is available. Ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) is an extensively used tool in metabolic profiling because of its high resolving power, analysis sensitivity, and selectivity. This technique allows for reliable structural elucidation for metabolite identification and quantification at low micromolar to nanomolar concentration levels. In this book chapter, we discuss two complementary UPLC-MS methods, reversed-phase (RP) and chromatography hydrophilic interaction liquid chromatography (HILIC), to characterize metabolomic analysis of CSF samples.

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Chromatographic Choice in Cerebrospinal Fluid Metabolomics Identification Outcome

  • 实验方案

  • 发表日期：

    2025年

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  • Sofía C. Ayala Rodríguez,
  • Alyssa M. Suarez,
  • Victor M. Lu,
  • Sanjoy K. Bhattacharya,
  • Ashish H. Shah

摘要

Limited literature on the optimal chromatographic method for cerebrospinal fluid (CSF) extraction and identification is available. Ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) is an extensively used tool in metabolic profiling because of its high resolving power, analysis sensitivity, and selectivity. This technique allows for reliable structural elucidation for metabolite identification and quantification at low micromolar to nanomolar concentration levels. In this book chapter, we discuss two complementary UPLC-MS methods, reversed-phase (RP) and chromatography hydrophilic interaction liquid chromatography (HILIC), to characterize metabolomic analysis of CSF samples.