Scholarlink

In this chapter, we outline the preparation and isolation of retinal ganglion cells (RGCs) from postnatal mice. This includes a metabolite extraction method using 1:1 methanol/water from 6 or 12-well plate dishes. We provide liquid chromatography-mass spectrometry (LC-MS) parameters using a QExactive High Resolution Orbitrap Mass Spectrometer and HILIC column. The software used for analysis includes Compound Discoverer and MetaboAnalyst. Through this metabolic workflow, this protocol provides a biomarker discovery, allowing for future experimentation to treat and cure ocular neuropathies.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Revealing the Metabolome: Metabolite Workflow for Retinal Ganglion Cells

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Alejandra Hernandez,
  • Sofia Yarosh,
  • Amr Almobayed,
  • Sanjoy K. Bhattacharya

摘要

In this chapter, we outline the preparation and isolation of retinal ganglion cells (RGCs) from postnatal mice. This includes a metabolite extraction method using 1:1 methanol/water from 6 or 12-well plate dishes. We provide liquid chromatography-mass spectrometry (LC-MS) parameters using a QExactive High Resolution Orbitrap Mass Spectrometer and HILIC column. The software used for analysis includes Compound Discoverer and MetaboAnalyst. Through this metabolic workflow, this protocol provides a biomarker discovery, allowing for future experimentation to treat and cure ocular neuropathies.