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Atmospheric pressure matrix-assisted laser desorption/ionization (AP/MALDI) mass spectrometry (MS) allows for highly sensitive and specific detection and relative quantification of metabolites of interest, with the key benefit of minimal sample preparation requirements for complex biological samples such as bacteria. Violacein is a violet-colored pigment produced by the bacterium Chromobacterium violaceum. In this chapter, we detail a high-throughput, high-resolution (HR) MS-based, targeted metabolite profiling protocol for analyzing violacein and its intermediates directly from bacterial colonies. This technique applies to a wide range of metabolites regulated by operon control and is suitable for a molecular phenotyping platform.

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Direct Colony Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Assay for Targeted Operon Metabolite Analysis

  • 实验方案

  • 发表日期：

    2025年

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  • Nivedita Bhattacharya,
  • Deepanwita Banerjee,
  • Caleigh O’Connor,
  • Dharmeshkumar Parmar,
  • Eugene Moskovets,
  • Peter D. E. M. Verhaert,
  • Anu Raghunathan,
  • Venkateswarlu Panchagnula

摘要

Atmospheric pressure matrix-assisted laser desorption/ionization (AP/MALDI) mass spectrometry (MS) allows for highly sensitive and specific detection and relative quantification of metabolites of interest, with the key benefit of minimal sample preparation requirements for complex biological samples such as bacteria. Violacein is a violet-colored pigment produced by the bacterium Chromobacterium violaceum. In this chapter, we detail a high-throughput, high-resolution (HR) MS-based, targeted metabolite profiling protocol for analyzing violacein and its intermediates directly from bacterial colonies. This technique applies to a wide range of metabolites regulated by operon control and is suitable for a molecular phenotyping platform.