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We have developed an image J macro to analyze pigmentation level in iPSC-derived retinal pigment epithelial cells from bright-field images. Human iPSC-derived retinal pigment epithelial cells were treated with hydroquinone to induce hyperpigmentation, and untreated cells were used as controls for the study. The Image analysis results were validated by estimating melanin spectrophotometrically, after lysing the cells. The developed image analysis method will be useful for screening drugs/compounds that induce hyper/hypo pigmentation in cultured iPSC-derived RPE cells.

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A Non-destructive, Image Analysis Method for Evaluating Pigmentation in Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells

  • 实验方案

  • 发表日期：

    2025年

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  • Gopika S. Kumar,
  • Gowri P. Chidambaranathan,
  • Anwar A. Palakkan

摘要

We have developed an image J macro to analyze pigmentation level in iPSC-derived retinal pigment epithelial cells from bright-field images. Human iPSC-derived retinal pigment epithelial cells were treated with hydroquinone to induce hyperpigmentation, and untreated cells were used as controls for the study. The Image analysis results were validated by estimating melanin spectrophotometrically, after lysing the cells. The developed image analysis method will be useful for screening drugs/compounds that induce hyper/hypo pigmentation in cultured iPSC-derived RPE cells.