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Loop-mediated isothermal amplification (LAMP) allows the detection of pathogens DNA by designing 4–6 primers that recognize 6–8 distinct regions of target DNA during an amplification reaction. Here we describe a novel microdroplet optofluidic system designed for the sensitive detection of foodborne pathogens, by integrating LAMP with surface-enhanced Raman scattering (SERS). This LAMP-on-a-chip offers the advantage of producing several LAMP reactions inside of the microdroplets using the same quantity of reagents in combination of an indirect SERS detection, utilizing multifunctional gold nanoparticles (AuNPs) and the formation of pyrophosphate generated during DNA amplification in LAMP. The microdroplet-LAMP-SERS methodology detected successfully down to 102.0 pg/μL of target DNA, which was equivalent ~102 CFU in 25 mL of UHT milk.

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LAMP-on-a-Chip in Combination with Surface-Enhanced Raman Scattering (SERS) for Pathogen Detection

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  • 发表日期：

    2025年

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  • Verónica Silva,
  • Bernardo A. Nogueira,
  • Sara Abalde-Cela,
  • Juan Luis Paris,
  • Laura Rodriguez-Lorenzo

摘要

Loop-mediated isothermal amplification (LAMP) allows the detection of pathogens DNA by designing 4–6 primers that recognize 6–8 distinct regions of target DNA during an amplification reaction. Here we describe a novel microdroplet optofluidic system designed for the sensitive detection of foodborne pathogens, by integrating LAMP with surface-enhanced Raman scattering (SERS). This LAMP-on-a-chip offers the advantage of producing several LAMP reactions inside of the microdroplets using the same quantity of reagents in combination of an indirect SERS detection, utilizing multifunctional gold nanoparticles (AuNPs) and the formation of pyrophosphate generated during DNA amplification in LAMP. The microdroplet-LAMP-SERS methodology detected successfully down to 102.0 pg/μL of target DNA, which was equivalent ~102 CFU in 25 mL of UHT milk.