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The critical public health risk caused by Salmonella necessitates the need for efficient detection methods. Conventional techniques based on plate counting and real-time polymerase chain reaction are time-consuming, costly, and labor-intensive, thus mainly performed in centralized laboratories. To meet the emerging need for decentralized/on-site testing, here we describe a rapid, low-cost, and simple method for Salmonella detection in coffee and cream by using gold nanoparticle-based loop-mediated isothermal amplification.

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Gold Nanoparticle-Based Loop-Mediated Isothermal Amplification for Rapid, Low-Cost, and Simple Detection of Salmonella in Coffee and Cream

  • 章节

  • 发表日期：

    2025年

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  • Shiyao Wang,
  • Eric Wai Him Lee,
  • Jack Yiu Tat Yuen,
  • Jackson Chit Shing Woo,
  • Stryer Wong,
  • Benjamin Wai Kit Chan,
  • Shea Ping Yip,
  • Thomas Ming Hung Lee

摘要

The critical public health risk caused by Salmonella necessitates the need for efficient detection methods. Conventional techniques based on plate counting and real-time polymerase chain reaction are time-consuming, costly, and labor-intensive, thus mainly performed in centralized laboratories. To meet the emerging need for decentralized/on-site testing, here we describe a rapid, low-cost, and simple method for Salmonella detection in coffee and cream by using gold nanoparticle-based loop-mediated isothermal amplification.