Thermal difference spectra (TDS) is a technique used to study the thermal stability and conformational changes of biomolecules by measuring the difference in absorbance at various temperatures. This protocol outlines the steps for setting up the system, generating TDS spectra, and performing the necessary calculations to analyze the data.

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Thermal Difference Spectra (TDS)

  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

Thermal difference spectra (TDS) is a technique used to study the thermal stability and conformational changes of biomolecules by measuring the difference in absorbance at various temperatures. This protocol outlines the steps for setting up the system, generating TDS spectra, and performing the necessary calculations to analyze the data.