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This protocol outlines the steps for performing Western Blot analysis on zebrafish embryos at 24 h post-fertilization (HPF), after yolk protein extraction. The process includes sample preparation, denaturing gel electrophoresis, electrotransfer to a nitrocellulose membrane, antibody incubation, and protein detection.

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Western Blot on Zebrafish Embryos

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

This protocol outlines the steps for performing Western Blot analysis on zebrafish embryos at 24 h post-fertilization (HPF), after yolk protein extraction. The process includes sample preparation, denaturing gel electrophoresis, electrotransfer to a nitrocellulose membrane, antibody incubation, and protein detection.