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This protocol describes the preparation and measurement of fluorescence using Thioflavin T (ThT) in a black microplate format. The fluorescence is measured by exciting ThT at 485 ± 20 nm and detecting emission at 528 ± 20 nm. ThT is used to study amyloid fibril formation and protein aggregation.

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Thioflavin T (ThT) Fluorescence Measurement

  • 实验方案

  • 发表日期：

    2025年

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  • Karuppusamy Arunachalam,
  • Puthanpura Sasidharan Sreeja

摘要

This protocol describes the preparation and measurement of fluorescence using Thioflavin T (ThT) in a black microplate format. The fluorescence is measured by exciting ThT at 485 ± 20 nm and detecting emission at 528 ± 20 nm. ThT is used to study amyloid fibril formation and protein aggregation.