Mesenchymal stem cells (MSCs) are multipotent stromal cells that have the ability to differentiate into various cell types, including adipocytes, chondrocytes, and osteoblasts. These differentiation protocols provide a comprehensive approach for inducing MSCs into specific lineages, with a focus on adipogenic, chondrogenic, and osteogenic differentiation. Together, these protocols provide a standardized approach for differentiating MSCs into specific lineages, facilitating studies in regenerative medicine, tissue engineering, and disease modeling.