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This chapter is intended to be a guide to skin and skin substitutes studies dealing with the analysis of subcellular structures of cells and tissues by providing an exhaustive sample preparation protocol for Transmission Electron Microscopy (TEM). Critical steps to obtain good ultrastructure are mentioned as well as notes to improve the sample collection. New ways to automatize the imaging acquisition are discussed.

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Standard Preparation Protocol of Human Skin and Skin Substitute Samples for Transmission Electron Microscopy

  • 实验方案

  • 发表日期：

    2025年

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  • Gery Barmettler,
  • Urs Ziegler

摘要

This chapter is intended to be a guide to skin and skin substitutes studies dealing with the analysis of subcellular structures of cells and tissues by providing an exhaustive sample preparation protocol for Transmission Electron Microscopy (TEM). Critical steps to obtain good ultrastructure are mentioned as well as notes to improve the sample collection. New ways to automatize the imaging acquisition are discussed.