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Lysine methyltransferases (KMTs) catalyze mono-, di-, and tri-methylation on histone and non-histone proteins. Determining the substrate specificity of KMTs is a critical task. This chapter outlines a method to perform highly sensitive in vitro methyltransferase assays on peptide substrates. In addition to comparing peptide substrates from histone or non-histone proteins, we also describe the use of a lysine-oriented peptide library (K-OPL) to generate high-resolution substrate specificity profiles for KMTs.

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Determining the Substrate Specificity of Lysine Methyltransferases

  • 实验方案

  • 发表日期：

    2025年

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  • Jocelyne N. Hanquier,
  • Christine A. Berryhill,
  • Evan M. Cornett

摘要

Lysine methyltransferases (KMTs) catalyze mono-, di-, and tri-methylation on histone and non-histone proteins. Determining the substrate specificity of KMTs is a critical task. This chapter outlines a method to perform highly sensitive in vitro methyltransferase assays on peptide substrates. In addition to comparing peptide substrates from histone or non-histone proteins, we also describe the use of a lysine-oriented peptide library (K-OPL) to generate high-resolution substrate specificity profiles for KMTs.