Scholarlink

Choroid plexus epithelial cells (CPECs) are responsible for the production of cerebrospinal fluid (CSF), which plays an important role in maintaining the milieu intérieur of the central nervous system. To elucidate the function of CPECs, in vitro primary culture is an ideal system as the choroid plexus (CP) is situated deep in the brain ventricular system in situ. This location makes detailed analysis of these cells difficult. Moreover, its highly undulating nature prevents quantitative study using molecular and cell biological tools. The protocols herein describe primary culture of CPECs in a differentiated state, in a well-integrated monolayer sheet that recapitulates the in vivo blood-cerebrospinal fluid barrier, to enable study of fluid transcytosis through the cytoplasm.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Transport Across the Choroid Plexus: How to Culture Choroid Plexus Epithelial Cells and Establish a Functional Assay System

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Sén Takeda,
  • Keishi Narita

摘要

Choroid plexus epithelial cells (CPECs) are responsible for the production of cerebrospinal fluid (CSF), which plays an important role in maintaining the milieu intérieur of the central nervous system. To elucidate the function of CPECs, in vitro primary culture is an ideal system as the choroid plexus (CP) is situated deep in the brain ventricular system in situ. This location makes detailed analysis of these cells difficult. Moreover, its highly undulating nature prevents quantitative study using molecular and cell biological tools. The protocols herein describe primary culture of CPECs in a differentiated state, in a well-integrated monolayer sheet that recapitulates the in vivo blood-cerebrospinal fluid barrier, to enable study of fluid transcytosis through the cytoplasm.