Cerebrospinal fluid (CSF) proteomics has become an alternative that allows zooming-in where pathophysiological alterations are taking place, detecting protein mediators that might eventually be considered as potential biomarkers in neurological or psychiatric diseases. From a technological point of view, mass-spectrometry-based-proteomics as well as antibody/aptamer-based platforms allow the simultaneous monitoring of secreted and circulating proteins in a broad concentration range in a robust manner, generating innovative facets in biomarker validation. This chapter highlights recent discoveries in the field of CSF protein analysis, covering sample preparation methods for proteomic workflows, shotgun proteomics, subproteomics, and other omics applied to CSF.