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The Mac-ELISA technique is based on the capture of IgM antibodies by the formation of an antigen-antibody complex in biological samples. IgM anti-yellow fever antibodies are captured by anti-IgM present on the polystyrene surface of the wells of the assay plate. The steps of the technique consist of stages (serum, antigen and anti-flavivirus conjugate - 6B6C-1), with incubation intervals at 37°C and washing. When adding the substrate in the next step, positive reactions (those containing the antigen-antibody complex) are blue in color by the action of the TMB substrate on the peroxidase enzyme. Completion of the reaction by adding the stop solution will cause the positive reaction of blue color to turn yellow.

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Immunoenzyme Test for Capture of IgM Anti-YFV Class Antibodies: MAC-ELISA

  • 实验方案

  • 发表日期：

    2025年

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  • Monique da Rocha Queiroz Lima,
  • Renata Monteiro-Maia,
  • Priscila Conrado Guerra Nunes

摘要

The Mac-ELISA technique is based on the capture of IgM antibodies by the formation of an antigen-antibody complex in biological samples. IgM anti-yellow fever antibodies are captured by anti-IgM present on the polystyrene surface of the wells of the assay plate. The steps of the technique consist of stages (serum, antigen and anti-flavivirus conjugate - 6B6C-1), with incubation intervals at 37°C and washing. When adding the substrate in the next step, positive reactions (those containing the antigen-antibody complex) are blue in color by the action of the TMB substrate on the peroxidase enzyme. Completion of the reaction by adding the stop solution will cause the positive reaction of blue color to turn yellow.