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The yellow fever virus’ ability to invade the host cell and replicate itself is an important property that allows us to quantify the load of infectious particles. The most-common method for quantifying the infectivity of cytopathic viruses is the plaque assay. The plaque assay quantifies the number of plaque-forming units (PFU) in a virus sample. Cell-based viral quantification assays are useful for viral diagnosis and in the different stages of vaccines development. This chapter will describe in detail a protocol to determine the potency of yellow fever virus in laboratory.

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Quantification of Yellow Fever Virus by Plaque Assay and Focus Formation Assay

  • 实验方案

  • 发表日期：

    2025年

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  • Stephanie Almeida da Silva,
  • Noemi Rovaris Gardinali,
  • Mariana Pierre de Barros Gomes

摘要

The yellow fever virus’ ability to invade the host cell and replicate itself is an important property that allows us to quantify the load of infectious particles. The most-common method for quantifying the infectivity of cytopathic viruses is the plaque assay. The plaque assay quantifies the number of plaque-forming units (PFU) in a virus sample. Cell-based viral quantification assays are useful for viral diagnosis and in the different stages of vaccines development. This chapter will describe in detail a protocol to determine the potency of yellow fever virus in laboratory.