Scholarlink

The yellow fever virus (YF), belonging to the Flaviviridae family, has an RNA genome of 10,862 nucleotides and continues to cause significant epidemics in Africa and South America despite an effective vaccine. Timely laboratory identification is essential for emergency immunization campaigns, but delays often lead to unconfirmed outbreaks. The presence of other diseases with similar symptoms complicates differential diagnosis, particularly in endemic regions, and the recent resurgence of YF activity has renewed interest in this arboviral disease. Genomic DNA (gDNA) and plasmids with cloned target sequences serve as standards in quantitative PCR, requiring specific calculations to determine their masses relative to target nucleic acid copy numbers.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

NS5 Region Cloning Protocol, for the Construction of an Accurate Standard Curve in the Detection and Diagnosis of the Yellow Fever Virus

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Alice Gomes Fernandes,
  • Gisela Freitas Trindade,
  • Vanessa S. de Paula,
  • Ana Cláudia Duarte,
  • Lecila Andrade Gomes,
  • Carolina dos Santos Messias,
  • Sheila Maria Barbosa de Lima

摘要

The yellow fever virus (YF), belonging to the Flaviviridae family, has an RNA genome of 10,862 nucleotides and continues to cause significant epidemics in Africa and South America despite an effective vaccine. Timely laboratory identification is essential for emergency immunization campaigns, but delays often lead to unconfirmed outbreaks. The presence of other diseases with similar symptoms complicates differential diagnosis, particularly in endemic regions, and the recent resurgence of YF activity has renewed interest in this arboviral disease. Genomic DNA (gDNA) and plasmids with cloned target sequences serve as standards in quantitative PCR, requiring specific calculations to determine their masses relative to target nucleic acid copy numbers.