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Immunofluorescence enables detection of proteins in tissue and cell culture. The specific reaction between the complex antigen-antibody confers security to detection. Disclosure of proteins of interest by fluorochrome allows a perception of its spatial distribution and location. Here we describe the technique of indirect immunofluorescence for detection of E protein from yellow fever virus in paraffinized tissue and in cell cultures.

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Detection of Viral Particles in Paraffinized Tissues and Cell Culture by Indirect Immunofluorescence

  • 实验方案

  • 发表日期：

    2025年

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  • Yuli Souza,
  • Barbara de Oliveira,
  • Marcelo Pelajo-Machado,
  • Pedro Paulo Manso

摘要

Immunofluorescence enables detection of proteins in tissue and cell culture. The specific reaction between the complex antigen-antibody confers security to detection. Disclosure of proteins of interest by fluorochrome allows a perception of its spatial distribution and location. Here we describe the technique of indirect immunofluorescence for detection of E protein from yellow fever virus in paraffinized tissue and in cell cultures.