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The tomato plant is the second most widely cultivated vegetable globally and a good model for studying fruit plants, as its genome is extensively sequenced. The Micro-Tom variety stands out as a valuable research tool due to its smaller size and shorter life cycle compared to other tomato varieties. This facilitates stable transformation from explants in a period of around 3 months to obtain shoots, with a high rate of successful transformation. Here, we present an effective and replicable methodology for the transformation of Micro-Tom cotyledons by means of Agrobacterium tumefaciens.

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Agrobacterium tumefaciens–Mediated Stable Cotyledon Transformation of Solanum lycopersicum var. Micro-Tom

  • 实验方案

  • 发表日期：

    2025年

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  • Daniela Arias-G,
  • Felipe Uribe

摘要

The tomato plant is the second most widely cultivated vegetable globally and a good model for studying fruit plants, as its genome is extensively sequenced. The Micro-Tom variety stands out as a valuable research tool due to its smaller size and shorter life cycle compared to other tomato varieties. This facilitates stable transformation from explants in a period of around 3 months to obtain shoots, with a high rate of successful transformation. Here, we present an effective and replicable methodology for the transformation of Micro-Tom cotyledons by means of Agrobacterium tumefaciens.