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B lymphocytes (B cells) are effector cells in humoral immunity. B cells recognize membrane-bound antigens through B-cell receptors in vivo, triggering signaling cascades and resulting in B-cell activation. During this process, cells exert myosin II-mediated traction to discriminate between different antigen densities and affinities. Traction force microscopy (TFM) allows for the quantitative measurement of dynamic traction generated by B cells. Experimental conditions must be optimized when conducting B-cell TFM tests. Here, we describe the general procedures for using TFM to profile the origin, dynamics, and the function of traction force during B-cell activation. Detailed experimental conditions have been listed, which instruct investigators to obtain high-quality TFM data for B-cell studies.

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Traction Force Microscopy for Studying B Lymphocyte Mechanosensing

  • 实验方案

  • 发表日期：

    2025年

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  • Yue Xu,
  • Chun Yang,
  • Yingyue Zeng,
  • Chenguang Xu,
  • Wanli Liu

摘要

B lymphocytes (B cells) are effector cells in humoral immunity. B cells recognize membrane-bound antigens through B-cell receptors in vivo, triggering signaling cascades and resulting in B-cell activation. During this process, cells exert myosin II-mediated traction to discriminate between different antigen densities and affinities. Traction force microscopy (TFM) allows for the quantitative measurement of dynamic traction generated by B cells. Experimental conditions must be optimized when conducting B-cell TFM tests. Here, we describe the general procedures for using TFM to profile the origin, dynamics, and the function of traction force during B-cell activation. Detailed experimental conditions have been listed, which instruct investigators to obtain high-quality TFM data for B-cell studies.