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The bacterial surfaceome, representing the full complement of surface-expressed proteins, is integral to interactions between bacteria, their hosts, and the environment, influencing processes such as adhesion, invasion, immune evasion, and biofilm formation. These proteins, due to their accessibility and vital roles in bacterial physiology, are prime targets for therapeutic intervention. This protocol describes a detailed methodology for mapping bacterial surfaceomes, including enzymatic surface shaving, cell surface biotinylation, solid-phase extraction, and liquid chromatography-tandem mass spectrometry.

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Updated Methods for Elucidating Bacterial Surfaceomes

  • 实验方案

  • 发表日期：

    2025年

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  • Veronica M. Jarocki,
  • Maria C. Rezcallah,
  • Pauline M. L. Coulon,
  • Matthew P. Padula,
  • Steven P. Djordjevic

摘要

The bacterial surfaceome, representing the full complement of surface-expressed proteins, is integral to interactions between bacteria, their hosts, and the environment, influencing processes such as adhesion, invasion, immune evasion, and biofilm formation. These proteins, due to their accessibility and vital roles in bacterial physiology, are prime targets for therapeutic intervention. This protocol describes a detailed methodology for mapping bacterial surfaceomes, including enzymatic surface shaving, cell surface biotinylation, solid-phase extraction, and liquid chromatography-tandem mass spectrometry.