Scholarlink

Larval zebrafish xenografts are a vertebrate model system suited for screening small compounds towards their anticancer effects. The small size and transparency of zebrafish larvae allow for phenotypic screening in 96-well format. Due to the achievable throughput in a living vertebrate organism, zebrafish xenografts promise to bridge the gap between in vitro screening and testing compounds in mouse xenografts. Here, we describe a workflow for establishing xenografts in zebrafish embryos/larvae for subsequent automated high-content imaging-based screening of small compounds.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

High-Content Imaging-Based Screening for Anticancer Compounds in Zebrafish Xenografts

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Caterina Sturtzel,
  • Sarah Grissenberger,
  • Andrea Wenninger-Weinzierl,
  • Martin Distel

摘要

Larval zebrafish xenografts are a vertebrate model system suited for screening small compounds towards their anticancer effects. The small size and transparency of zebrafish larvae allow for phenotypic screening in 96-well format. Due to the achievable throughput in a living vertebrate organism, zebrafish xenografts promise to bridge the gap between in vitro screening and testing compounds in mouse xenografts. Here, we describe a workflow for establishing xenografts in zebrafish embryos/larvae for subsequent automated high-content imaging-based screening of small compounds.