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Overexpressing a gene in a mammalian cell line can be an extremely powerful technique to investigate and explore a gene’s biological functions and associated pathways. Here we describe how combining wet-lab methodology and in-silico transcriptomic approaches can aid in the discovery of unknown gene functions, perturbation of molecular processes or the elucidation of mechanism of action.

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Exploring the Perturbation of Biological Processes Caused by Gene Upregulation Using Knock-In Transfection, Transcriptomics, and Bioinformatics Analysis

  • 实验方案

  • 发表日期：

    2025年

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  • Isabella R. Garcia,
  • Lee D. Larcombe

摘要

Overexpressing a gene in a mammalian cell line can be an extremely powerful technique to investigate and explore a gene’s biological functions and associated pathways. Here we describe how combining wet-lab methodology and in-silico transcriptomic approaches can aid in the discovery of unknown gene functions, perturbation of molecular processes or the elucidation of mechanism of action.