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NFAT1 translocation to the nucleus and consequent IL-2 production in T cells can be used as a downstream read-out for initial T cell activation via the T cell receptor complex. Here, we describe a protocol for the detection of NFAT1 translocation using anti-CD3/anti-CD28 coated beads as a stimulus of primary murine CD4+ T cells by immunohistochemistry. Further, an open-source Python code is described to determine NFAT1 translocation.

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Visualization and Quantification of NFAT1 Translocation in T Cells

  • 实验方案

  • 发表日期：

    2025年

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  • Franziska Möckl,
  • Dejan Kovacevic,
  • René Werner,
  • Björn-Philip Diercks,
  • Mariella Weiß

摘要

NFAT1 translocation to the nucleus and consequent IL-2 production in T cells can be used as a downstream read-out for initial T cell activation via the T cell receptor complex. Here, we describe a protocol for the detection of NFAT1 translocation using anti-CD3/anti-CD28 coated beads as a stimulus of primary murine CD4+ T cells by immunohistochemistry. Further, an open-source Python code is described to determine NFAT1 translocation.