The bead-based luminescent Alpha technology is a fast, highly sensitive, and robust method for studying DNA-binding activities of the transcription factors NF-kappaB and AP-1 in human or murine T cells. It represents a reproducible and time-efficient technology, which can replace classical EMSA method and is thus an ideal tool in basic research for the study of cellular transcriptional regulatory responses.

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Analysis of TF/DNA Binding in T Cells Applying Alpha Technology

  • Nikolaus Thuille,
  • Gottfried Baier

摘要

The bead-based luminescent Alpha technology is a fast, highly sensitive, and robust method for studying DNA-binding activities of the transcription factors NF-kappaB and AP-1 in human or murine T cells. It represents a reproducible and time-efficient technology, which can replace classical EMSA method and is thus an ideal tool in basic research for the study of cellular transcriptional regulatory responses.