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Cytotoxic CD8+ T cell lymphocytes are pivotal for fighting infections with intracellular pathogens, e.g., viruses. Moreover, recent studies have elucidated the role of CD8+ T cells in various neuroinflammatory diseases such as Alzheimer’s disease (AD), Parkinson’s disease (PD), multiple sclerosis (MS), and stroke. Therefore, the characterization of brain CD8+ T cells at the single-cell level is pivotal for understanding their state and function, e.g., in preclinical mouse models. Here, flow cytometry emerges as a versatile tool offering distinct techniques tailored to different facets of T cell activation. In this chapter, we provide protocols utilizing multicolor flow cytometry and cytokine secretion assays to analyze brain CD8+ T cell activation via the detection of surface lineage markers, extracellular and intracellular activation markers such as CD39, CD279, and granzyme B, or via measuring cytokine expression levels.

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Flow Cytometric Analysis of Mouse Brain CD8+ T Cell Activation

  • 实验方案

  • 发表日期：

    2025年

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  • Du Huy Nguyen,
  • Manja Wibke Thiem,
  • Alexander Winston Veltkamp,
  • Ines Sophie Schädlich,
  • Jiani Jiang,
  • Mamta Panda,
  • Enrico Maria Costanzo,
  • Tim Magnus,
  • Björn Rissiek

摘要

Cytotoxic CD8+ T cell lymphocytes are pivotal for fighting infections with intracellular pathogens, e.g., viruses. Moreover, recent studies have elucidated the role of CD8+ T cells in various neuroinflammatory diseases such as Alzheimer’s disease (AD), Parkinson’s disease (PD), multiple sclerosis (MS), and stroke. Therefore, the characterization of brain CD8+ T cells at the single-cell level is pivotal for understanding their state and function, e.g., in preclinical mouse models. Here, flow cytometry emerges as a versatile tool offering distinct techniques tailored to different facets of T cell activation. In this chapter, we provide protocols utilizing multicolor flow cytometry and cytokine secretion assays to analyze brain CD8+ T cell activation via the detection of surface lineage markers, extracellular and intracellular activation markers such as CD39, CD279, and granzyme B, or via measuring cytokine expression levels.