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The ability to titer virus is critical for quantifying the efficacy of interventions on viral replication as well as for monitoring the consistency and viability of viral stocks to ensure reproducible experiments. Infectious rhinovirus is quantified by determining the dilution of virus that infects and causes cytopathic effects in a permissive cell line. Here we outline a protocol for determining tissue culture infectious dose 50 (TCID50) of rhinovirus-16 in WI-38 fibroblasts using an adapted microtiter plate system.

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Assessment of Viral Replication in Rhinovirus-Infected Airway Epithelial Cells

  • 实验方案

  • 发表日期：

    2025年

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  • Michelle E. Love,
  • Shahina Wiehler,
  • David Proud

摘要

The ability to titer virus is critical for quantifying the efficacy of interventions on viral replication as well as for monitoring the consistency and viability of viral stocks to ensure reproducible experiments. Infectious rhinovirus is quantified by determining the dilution of virus that infects and causes cytopathic effects in a permissive cell line. Here we outline a protocol for determining tissue culture infectious dose 50 (TCID50) of rhinovirus-16 in WI-38 fibroblasts using an adapted microtiter plate system.