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H1HeLa cell line has become the most utilized cell culture system for in vitro studies of the rhinovirus life cycle. These cells are also widely used to propagate the rhinovirus to study host responses. Unlike other viruses, high-titer virus is required for studies focusing on understanding the host responses to rhinovirus infection in vivo and in vitro. Therefore, after propagation of rhinovirus, H1HeLa cells should be concentrated and purified. In this chapter, we describe the methods used for the propagation of rhinovirus A and B that are adapted to H1HeLa cells.

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Laboratory Protocol for Propagation and Purification of Rhinovirus A and B Suitable for In Vitro and In Vivo Infection

  • 实验方案

  • 发表日期：

    2025年

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  • Haleh Ganjian,
  • Umadevi Sajjan

摘要

H1HeLa cell line has become the most utilized cell culture system for in vitro studies of the rhinovirus life cycle. These cells are also widely used to propagate the rhinovirus to study host responses. Unlike other viruses, high-titer virus is required for studies focusing on understanding the host responses to rhinovirus infection in vivo and in vitro. Therefore, after propagation of rhinovirus, H1HeLa cells should be concentrated and purified. In this chapter, we describe the methods used for the propagation of rhinovirus A and B that are adapted to H1HeLa cells.