Rhinovirus is a prevalent respiratory pathogen responsible for a significant proportion of upper and lower respiratory tract infections. Its ability to cause a range of illnesses, from mild common cold symptoms to more severe respiratory diseases, underscores the importance of accurate and timely detection. Detecting rhinovirus in clinical samples is an important diagnostic process for identifying and diagnosing respiratory infections caused by this common virus. This chapter of the book provides a comprehensive overview of the methodologies and techniques employed in the detection of rhinovirus in clinical samples.