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Most mammalian cells cannot survive freeze-drying treatment. However, mammalian nuclei can obtain strong tolerance against environmental changes after freeze-drying treatment. Using this method, it is now possible to produce offspring from dead cells via sperm injection or cloning techniques, even when the cells have been preserved at room temperature for long periods of time. This is a safe and low-cost method for preserving genetic resources. In this chapter, we introduce a freeze-drying and preservation method for mammalian spermatozoa.

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Production of Healthy Mice from Freeze-Dried Spermatozoa Preserved at Room Temperature

  • 实验方案

  • 发表日期：

    2025年

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  • Sayaka Wakayama,
  • Daiyu Ito,
  • Teruhiko Wakayama

摘要

Most mammalian cells cannot survive freeze-drying treatment. However, mammalian nuclei can obtain strong tolerance against environmental changes after freeze-drying treatment. Using this method, it is now possible to produce offspring from dead cells via sperm injection or cloning techniques, even when the cells have been preserved at room temperature for long periods of time. This is a safe and low-cost method for preserving genetic resources. In this chapter, we introduce a freeze-drying and preservation method for mammalian spermatozoa.