Detection of Sperm Membrane Proteins by Immunocytochemistry, Scanning and Transmission Electron Microscopy, and Western Blotting
摘要
Western blotting in combination with scanning and transmission electron microscopy provide accurate and complementary information on the presence and localization of proteins in the plasma membrane, as well as the structural and ultrastructural features of sperm cells. The present chapter describes the procedures to detect the presence of membrane proteins by Western blotting and to localize them in the plasma membrane of sperm cells.