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In sperm, oxidative stress triggers structural damage to biomolecules, DNA, lipids, carbohydrates, and proteins, as well as other cellular components that eventually impair multiple signaling pathways resulting in male sub/infertility. In this chapter, we describe the methods applied to evaluate the effects of the oxidative stress on sperm attributes of domestic and wild small ruminants’ species, mainly by flow cytometry. Together with the traditional usage of fluorescent probes, we provide details of the knowhows involving the combination of immunodetection and the flow cytometry technologies.

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Oxidative Status Measurement in Domestic and Wild Small Ruminants’ Species

  • 实验方案

  • 发表日期：

    2025年

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  • Olga García-Álvarez,
  • Ana Josefa Soler,
  • María Iniesta-Cuerda,
  • María del Rocío Fernández-Santos,
  • Felipe Martínez-Pastor,
  • Enrique del Olmo,
  • Pedro Javier Soria-Meneses,
  • Alejandro Jurado-Campos,
  • Alejandro Maroto-Morales,
  • Patricia Peris-Frau,
  • Vidal Montoro,
  • Jose Julián Garde

摘要

In sperm, oxidative stress triggers structural damage to biomolecules, DNA, lipids, carbohydrates, and proteins, as well as other cellular components that eventually impair multiple signaling pathways resulting in male sub/infertility. In this chapter, we describe the methods applied to evaluate the effects of the oxidative stress on sperm attributes of domestic and wild small ruminants’ species, mainly by flow cytometry. Together with the traditional usage of fluorescent probes, we provide details of the knowhows involving the combination of immunodetection and the flow cytometry technologies.