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Cryopreservation is the procedure that allows preserving cells, including gametes, for long time periods while maintaining their functionality, through the use of cryogenic temperature. Currently, sperm cryopreservation is a crucial-assisted reproductive biotechnology in pigs since it is the only effective and efficient method for long-term preservation of boar fertility. Here, we describe a two-step cryopreservation protocol for boar sperm using lactose-egg yolk and glycerol-based extenders.

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Sperm Cryopreservation in Boars

  • 实验方案

  • 发表日期：

    2025年

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  • Inmaculada Parrilla,
  • María A. Gil,
  • Cristina Cuello,
  • Emilio A. Martinez

摘要

Cryopreservation is the procedure that allows preserving cells, including gametes, for long time periods while maintaining their functionality, through the use of cryogenic temperature. Currently, sperm cryopreservation is a crucial-assisted reproductive biotechnology in pigs since it is the only effective and efficient method for long-term preservation of boar fertility. Here, we describe a two-step cryopreservation protocol for boar sperm using lactose-egg yolk and glycerol-based extenders.