Thermal Inactivation of Plant Viruses
摘要
Thermal inactivation point (TIP) of viruses serves as an important aspect in understanding the physicochemical properties of different viruses and the functioning of different components in a virus particle (Hahon and Kozikowski, J Bacteriol 81:609–613, 1961). Depending on the nature and topology of the virus, the thermal activation point will differ (Dijkstra et al, Practical plant virology: protocols and exercises, 1998). This protocol offers a simple and efficient methodology by lowering the temperature by 5 °C to test the thermal inactivation point of different plant viruses.